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Compréhension des mécanismes d'adhésion des biofilms en milieu marin en vue de la conception de nouveaux moyens de prévention ArchiMer
Rubio, Celine.
Biofilm formation on surfaces immersed in sea water induces equipment damages. Recently, studies focused on the environmental impact of antifouling paints have shown that some active chemical compounds are harmful to the aquatic environment. Hence, it appears fundamental to study physico-chemical interactions existing between marine bacteria and various substrata to conceive new ecological products or antifouling processes. First, we model an artificial primary organic film, formed by the adsorption of BSA and polysaccharide, as close as the one formed in natural seawater. This organic film is heterogeneous (only 42% of the surface is covered) and organic adsorption occurs in a multi-layer scheme, molecules bounded each others by calcium and magnesium...
Tipo: Text Palavras-chave: Sea water; Marine bacteria; Physico chemical characterisation; MATS; XPS; IRAS; Adsorption; Bacterial adhesion; Biofim; Eau de mer; Bactéries marines; Caractérisation physicochimique; MATS; XPS; IRRAS; Adsorption; Adhésion bactérienne; Biofilm.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/these-222.pdf
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Evaluation of ultraviolet radiation to control microorganisms adhering to low-density polyethylene films BJM
Silva,Cleuber Antonio de Sá; Andrade,Nélio José de; Soares,Nilda de Fátima Ferreira; Ferreira,Sukarno Olavo.
Efficiency of ultraviolet (UV) radiation in reducing the cell number of Staphylococcus aureus ATCC 25923 and Escherichia coli K-12 adhered to low-density polyethylene (LDPE) films was evaluated. The microorganisms were let to adhere to the surface of LPDE bags for 12h at 18ºC, and then submitted to UV radiation at an intensity 196 µW.cm-2, 254nm, for 2 seconds. Staphylococcus aureus was less resistant to UV radiation than E. coli, and the efficiency increased with the increase of the concentration of microbial suspension. After 1500 hours of use the UV radiation intensity of the lamp was reduced from 288 to 78 µW.cm-2, and the higher decrease occurred in the first 100 hours of use. Also, the efficiency of the UV radiation decreased after 1500 hours of use....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adhesion; Ultraviolet radiation; Low-density polyethylene; Atomic force microscopy.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200017
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In vitro inhibition of adhesion of Escherichia coli strains by Xylitol BABT
Silva,Annelisa Farah da; Suzuki,Érika Yoko; Ferreira,Aline Siqueira; Oliveira,Murilo Gomes; Silva,Sílvio Silvério da; Raposo,Nádia Rezende Barbosa.
The present study aimed to evaluate xylitol's antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scanning electron microscopy (SEM) demonstrated that the slides treated with xylitol had a significant reduction in the number of bacilli and the inhibition of microbial adhesion was probably the xylitol's mechanism of action. Xylitol could be a possible alternative on the control of E. coli infections.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adhesion; Escherichia coli; Scanning electron microscopy; Xylitol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200003
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In vitro inhibition of Pseudomonas aeruginosa adhesion by Xylitol BABT
Sousa,Letícia Pinheiro de; Silva,Annelisa Farah da; Calil,Natalia Oliveira; Oliveira,Murilo Gomes; Silva,Silvio Silvério da; Raposo,Nádia Rezende Barbosa.
This study evaluated, in vitro, the antimicrobial activity and the anti-adherent property of xylitol (0.5, 2.5 and 5.0%, w/v) on two Pseudomonas aeruginosa strains (ATCC 9027 and clinical). The assay of antimicrobial activity was performed to determine a minimum inhibitory concentration (MIC) and the adhesion test was performed, by which the parameters regarding, growth in the culture medium, number of colony forming units (CFUs) released and slide evaluation by scanning electron microscopy (SEM) were analyzed. The Statistical Package for the Social Sciences (SPSS) was employed for statistical analysis. Results showed that xylitol had no antimicrobial activity on these strains; however, the inhibition of bacterial adherence was observed in microphotographs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adhesion; Pseudomonas aeruginosa; Prevention and control; Xylitol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500004
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Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces BABT
Zeraik,Ana Eliza; Nitschke,Marcia.
Bacterial adhesion to inert surfaces is a complex process influenced by environmental conditions. In this work, the influence of growth medium and temperature on the adhesion of Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes to polystyrene surfaces was studied. Most bacteria demonstrated the highest adhesion when cultured in TSYEA, except S. marcescens, which showed to be positively influenced by the pigment production, favored in poor nutrient media (lactose and peptone agar). P. aeruginosa adhesion to polystyrene increased at low temperatures whatever the medium used. The culture medium influenced the surface properties of the bacteria as assessed by the MATS test.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adhesion; Biofilms; Hydrophobicity; Polystyrene.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400012
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Nitrogen starvation affects bacterial adhesion to soil BJM
Borges,Maria Tereza; Nascimento,Antônio Galvão; Rocha,Ulisses Nunes; Tótola,Marcos Rogério.
One of the main factors limiting the bioremediation of subsoil environments based on bioaugmentation is the transport of selected microorganisms to the contaminated zones. The characterization of the physiological responses of the inoculated microorganisms to starvation, especially the evaluation of characteristics that affect the adhesion of the cells to soil particles, is fundamental to anticipate the success or failure of bioaugmentation. The objective of this study was to investigate the effect of nitrogen starvation on cell surface hydrophobicity and cell adhesion to soil particles by bacterial strains previously characterized as able to use benzene, toluene or xilenes as carbon and energy sources. The strains LBBMA 18-T (non-identified), Arthrobacter...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrophobicity; Bacterial adhesion; Nitrogen starvation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300009
Registros recuperados: 6
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